108 Colorimetric Method for the Determination of Alpha-amylase Activity

Methods Type: Generic Methods
Key data
Number: 108
Analyte: Alpha-amylase Activity (Colorimetric Method)
Matrix: Cereals or Cereal Products
Year of Approval: 1968
Scope: The method is applicable to cereals or cereal products ranging from very low to very high in alpha-amylase activity. It can also be used for estimating the alpha-amylase activity of additives of fungal and bacterial origin.
Principle: The decrease with time of the intensity of colour obtained with the diluted iodine solution is used as an index of starch degradation.


Alpha-amylase activity is expressed as a function of alpha-amylase concentration and of the velocity constant for the hydrolytic degradation of limit dextrin.