112 Microbiological Assay of Nicotinic Acid in Cereal Products

Methods Type: Generic Methods
Key data
Number: 112
Analyte: Nicotinic Acid (Microbiological Assay)
Matrix: Cereal Products
Year of Approval: 1972
Scope: The following method is applicable to grains, flours, cereal and starch products.
Principle: The method is based on the observation that Lactobacillus plantarum requires nicotinic acid (or nicotinamide) for growth. Using a basal medium complete in all respects except for nicotinic acid, growth responses of the organism are compared quantitatively in standard and unknown solutions. The acid produced by the organism is measured to determine the extent of growth and thereby the amount of vitamin in the test solution.


The method makes use of Lactobacillus plantarum (ATCC 8014) as the organism for analysis This organism responds to nicotinic acid, nicotinamide, and nicotinuric acid. The method does not attempt to distinguish which of these compounds is responsible for the vitamin activity of the sample.


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