Principle: Samples (duplicates at least, but preferably two duplicates), defatted if necessary (see section 7.1), are gelatinized in the presence of heat stable alpha amylase, and then enzymatically digested with protease and amyloglucosidase to remove digestible protein and starch. Four volumes of ethanol are added to precipitate soluble dietary fibre. Total residue is filtered off and washed with ethanol and acetone. The residue is weighed after drying. The remaining material is analysed for protein and ash content, respectively. Subtracting the amounts measured for protein, ash and a blank control from the dry weight of the filtered residue yields a value for total dietary fibre content.