105/2 Determination of Crude Protein in Cereals and Cereal Products for Food and for Feed

Methods Type: Generic Methods
Key data
Number: 105/2
Analyte: Crude Protein
Matrix: Cereals and Cereal Products for Food and for Feed
Year of Approval: 1980
Year of Last Revision: 1994
Scope: This method is envisaged for the determination of crude protein content in cereals and cereal products.
Principle: The organic matter of the sample is oxidized with concentrated sulfuric acid in the presence of a catalyst: the product of the reaction (NH4)2SO4 is treated by alkali; free ammonia is distilled and titrated.


Crude protein is a conventional expression for the total content of nitrogenous compounds of the analysed product, calculated by multiplying the corresponding total nitrogen content by an conventional factor.


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